Janet S. Lee, MD
Professor of Medicine

Dr. Lee
Dr. Lee is Professor of Medicine, University of Pittsburgh School of Medicine.

Office Location:
UPMC Montefiore Hospital - NW628
3459 Fifth Avenue
Pittsburgh, PA 15213

Contact Information:
Office Telephone: 412-692-2210
Fax: 412-692-2260
Email: leejs3@upmc.edu

Education

  • BA - 1991, The Johns Hopkins University
  • MD - 1995, Georgetown University School of Medicine

Clinical Interests

Broadly speaking, our laboratory studies pulmonary host defense and molecular pathogenesis of acute lung injury. Our research focuses upon the innate arm of immunity, specifically examining effector function of myeloid cells such as macrophages and neutrophils and how they recognize and respond to exogenous pathogen associated molecular patterns or endogenous alarmins. We are interested in probing host-pathogen interactions to examine mechanisms of host protection following pathogen-triggered injury from products of extracellular gram-negative pathogens such as Pseudomonas aeruginosa and Klebsiella pneumoniae. We are also interested in the factors that shape repair and resolution following injury, specifically factors derived from hematopoietic cells such as platelets and red blood cells that can influence the course of inflammation. We utilize a repertoire of relevant murine models of injury, molecular genetic approaches, in vitro biochemical assays, and human bio-samples to examine innate host defenses of the lung.

Selected Publications

  1. Bain W, Olonisakin T, Yu M, Qu Y, Hulver M, Xiong Z, Li H, Pilewski J, Mallampalli R, Ray A, Ray P, Cheng Z, Shanks R, Alexander W, St. Croix C, Silverstein R, Lee JS. Platelets inhibit apoptotic lung epithelial cell death and protect mice against infection-induced lung injury. Blood Adv. 2019; Feb 12;3(3): 432-445.
  2. Dar HH, Tyurina Y, Mikulska-Raminska K, Shrivastava I, Tyurin V, Krieger J, St. Croix C, Watkins S, Bayir E, Flitter R, Freedman C, Holman T, Pilewski J, Greenberger J, Mallampalli R, Doi Y, Lee JS, Bahar I, Bomberger J, Kagan V. Pseudomonas aeruginosa utilizes host polyunsaturated phosphatidylethanolamines to trigger theft-ferroptosis in bronchial epithelium. J Clin Invest. 2018; Oct 1;128(10): 4639-4653.
  3. Qu Y, Olonisakin T, Bain W, Zupetic J, Brown R, Hulver M, Xiong Z, Tejero J, Shanks RMQ, Bomberger JM, Cooper VS, Zegans ME, Ryu H, Han J, Pilewski J, Ray A, Cheng Z, Ray P, Lee JS. Thrombospondin-1 protects against pathogen-induced lung injury by limiting extracellular matrix proteolysis. JCI Insight. 2018; Feb 8;3(3): PMID:29415890.
  4. Chakraborty K, Raundhal M, Chen B, Morse C, Tyurina YY, Khare A, Oriss TB, Huff R, Lee JS, St. Croix CM, Watkins S, Mallampalli RK, Kagan V, Ray A, Ray P. The Mito-DAMP Cardiolipin Blocks IL-10 Production Causing Persistent Inflammation During Bacterial Pneumonia. Nature Communications. 2017; Jan 11;8: 13944.
  5. Flitter BA, Hvorecny KL, Ono E, Eddens T, Yang J, Kwak DH, Bahl CD, Hampton T, Morisseau C, Hammock BD, Liu X, Lee JS, Kolls JK, Levy BD, Madden DR, Bomberger JM. Pseudomonas aeruginosa sabotages the generation of host pro-resolving lipid mediators. Proc Natl Acad Sci USA. 2016; Dec 15: Epubaheadofprint.
  6. Olonisakin T, Li HH, Xiong Z, Kochman EJK, Yu MT, Qu Y, Hulver M, Kolls JK, St. Croiz C, Doi Y, Nguyen MH, Shanks RMQ, Mallampalli RK, Kagan VE, Ray A, Silverstein RL, Ray P, Lee JS. CD36 provides host protection against Klebsiella pneumoniae intrapulmonary infection by enhancing LPS responsiveness and macrophage phagocytosis. J Infectious Dis. 2016; Sep 28: Epubaheadofprint.
  7. Chen K, Eddens T, Trevejo-Nunez G, Way EE, Elsegeiny W, Ricks DM, Garg AV, Erb CJ, Bo M, Wang T, Chen W, Lee JS, Gaffen SL, Kolls JK. Epithelial IL-17R signaling is required for mucosal chemokine gradients and pulmonary host defense against K. pneumoniae. Cell Host & Microbe. 2016; Nov 9;20(5): 596-605.
  8. Zhao Y, Olonisakin TF, Xiong Z, Hulver M, Sayeed S, Yu MT, Gregory AD, Kochman EJ, Chen BB, Mallampalli RK, Sun M, Silverstein RL, Stolz DB, Shapiro SD, Ray A, Ray P, Lee JS. Thrombospondin-1 regulates neutrophil microbial killing by restraining granule serine proteases during K. pneumoniae infection. Mucosal Immunol. 2015; Jul;8(4): 896-905.
  9. Zhao Y, Xiong Z, Lechner EJ, Chan Y, Zhang Y, Ross MA, Stolz DB, Rosengart MR, Pilewski J, Ray P, Ray A, Silverstein RL, Lee JS. Thrombospondin-1 triggers macrophage IL-10 production and promotes resolution of experimental lung injury. Mucosal Immunol. 2014; March;7(2): 440-8.
  10. Mangalmurti NS, Xiong Z, Hulver M, Ranganathan M, Liu XH, Oriss T, Rubin M, Triulzi D, Choi A, Lee JS. Loss of Red cell Chemokine Scavenging Promotes Transfusion Related Lung Inflammation. Blood. 2009; January 29; 113(5): 1158-66.