Flow Cytometry -- Mesentric Adenopathy


Cell suspension immunophenotypic studies were performed on an enlarged right iliac lymph node and two regions were analyzed.

Region 1 (R1) represents the small noncomplex cells (71 % of the events).
Region 2 (R2) represents the larger noncomplex cells (9 % of the events).


B Cells
Antigen Usual Specificity % Positive R1 % Positive R2
Kappa + B Cell Subset 8 6
Lambda + B Cell Subset 7 5
Kappa:Lambda Ratio 1.1 1.2
CD19+ B Cell 24 27
CD19+/CD5+ B Cell Subset 3 15
CD20+ B Cell 16 11
CD10+ (Calla) B Cell Subset 3 3

T/NK Cells
Antigen Usual Specificity % Positive R1 % Positive R2
CD2+ T Cell 74 73
CD3+ T Cell 76 70
CD5+/CD19- T Cell 75 58
CD7+ T Cell 75 80
CD4+/CD8- T Helper 37 42
CD8+/CD4- T Cytotoxic/Suppressor 31 20
CD4:CD8 Ratio T Helper:Suppressor Ratio 1.2 2.1
CD16,CD56+/CD3+ T Cell Subset 11 22
CD16,CD56+/CD3- NK Cells 2 11

Additional Antibodies
Antigen Usual Specificity % Positive R1 % Positive R2
CD45+ Leukocytes 100 100
CD43+/CD19+ B Cell Subset 1 5
CD14+ Mono, Histiocytes 11 33


Flow cytometric immunophenotypic studies performed on the right iliac lymph node do not characterize the Reed-Sternberg-like cells because the cells are not well represented in the cell suspension. The lymphoid cells studied demonstrate polyclonal B-cells and a greater number of heterogeneous T-cells. The large cells include some histiocytes and show some nonspecific staining. These results do not further define the precise nature of the patient's posttransplant lymphoproliferative disorder.

Contributed by Steven H. Swerdlow, MD

Molecular Diagnostics


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