Flow Cytometry -- Lymphadenopathy


Results: Cell suspension immunophenotypic studies were performed on the bone marrow aspirate and one region was analyzed.

Region 1 represents the small non-complex cells (52% of the events).


B Cells
Antigen Usual Specificity % Positive
Kappa + B Cell Subset 2
Lambda + B Cell Subset 3
Kappa:Lambda Ratio 0.7
CD19+ B Cell 11
CD19+/CD5+ B Cell Subset 3
CD20+ B Cell 6
CD10+ (Calla) B Cell Subset 3

T Cells
Antigen Usual Specificity % Positive
CD2+ T Cell 84
CD3+ Total T Cell 78
CD5+/CD19- T Cell 74
CD7+ T Cell 72
CD4+/CD8- T Helper 13
CD8+/CD4- C Cytotoxic/Suppressor 63
CD4+/CD8+ T Cell Subset 1
CD4:CD8 Ratio T Helper:Suppressor Ratio 0.2
CD16,CD56+/CD3+ T Cell Subset 2
CD16,CD56+/CD3- NK Cells 7

Antigen Usual Specificity % Positive
CD15+ Myeloid 4
CD34+ Stem Cell 4

Additional Antibodies
Antigen Usual Specificity % Positive
CD45+ Leukocytes 96
HLA DR+ B, Myeloid, Activated T 78
TdT+ Lymphoblasts (Myeloblasts) 2
Cyto CD3+ T Cells 76
CD22+ 2


Flow cytometric immunophenotypic studies reveal an increased population of CD3+ T-lymphocytes (78%). This poulation also showed a low CD4:CD8 ratio (0.2) and strong expression of HLA-DR antigen (78%). These findings are consistent with infectious mononucleosis.

Final Diagnosis


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