BIOCHEMICAL TESTS:
The smaller colonies:
Biochemical tests were performed using the Rapid NFT strip (BioMe'rieux Vitek, Inc. Missouri, USA) on the organisms from the smaller colony. The results were:
TESTS PERFORMED | REACTION TESTED | RESULTS | |
NO3 | Reduction of nitrates to nitrites | POSITIVE | |
TRP | Indole production | POSITIVE | |
GLU | Acidi production from Glucose | POSITIVE | |
ADH | Arginine hydrolysis | NEGATIVE | |
URE | Urease | NEGATIVE | |
ESC | Hydrolysis (b-glucosidase) | NEGATIVE | |
GEL | Hydrolysis (protease) | NEGATIVE | |
PNPG | b-galactosidase | NEGATIVE | |
GLU | Assimilation | NEGATIVE | |
ARA | Assimilation | NEGATIVE | |
MNE | Assimilation | NEGATIVE | |
MAN | Assimilation | NEGATIVE | |
NAG | Assimilation | NEGATIVE | |
MAL | Assimilation | NEGATIVE | |
GNT | Assimilation | NEGATIVE | |
CAP | Assimilation | NEGATIVE | |
ADI | Assimilation | NEGATIVE | |
MLT | Assimilation | NEGATIVE | |
CIT | Assimilation | NEGATIVE | |
PAC | Assimilation | NEGATIVE |
The other tests performed on the smaller colonies included the triple sugar iron agar test (A/K), the oxidase test, catalase test, ornithine decarboxylase test and the indole test which were all positive. The urease test however was negative.