TRANSFUSION REACTION WORKUP:
Pre-transfusion:
The patient was A positive on pre-transfusion ABO/Rh typing:
| Forward | Reverse | Rh | ABO/Rh | ||
| Anti-A | Anti-B | Cell A | Cell B | anti-D | A POS |
| 4+ | - | - | 4+ | 3+ | |
His antibody screen was negative
Six hours Post-transfusion:
Urine hemoglobin was negative. Visual plasma hemoglobin (VISHGB) was negative. Posttransfusion ABO/Rh typing of the patient's serum showed a weakly positive reaction with A Cells.
| Forward | Reverse | Rh | ||
| Anti-A | Anti-B | Cell A | Cell B | anti-D |
| 4+ | - | w+ | 4+ | 3+ |
Direct Antiglobuin Test (DAT) showed weak positivity using polyspecific and anti-IgG reagents and microscopic positivity using anti-C3d.
|
Direct Antiglobulin Test |
||
|
Polyspcific |
anti-IgG |
anti-C3d |
|
1+ (weak) |
1+ |
M |
Rapid acid elution was performed on the postransfusion specimen. Antibody identification panel on the eluate showed anti A antibodies reacting at AGT(2+)
| Immediate Spin | 37º | IgG AGT | |
| Group O Cell I | - | - | - |
| Group O Cell II | - | - | - |
| Group A Cell | - | - | 2+ |
| Group B Cell | - | - | - |
|
