A Gram and an acid-fast stain were performed on the submitted specimen, and no organisms were seen. Routine and fungal cultures were negative. However, the mycobacterial broth culture produced growth of acid-fast organisms after fourteen days. On Ziehl-Neelson acid fast stain the cultured organism appeared as moderately elongated rods, some with a banded staining pattern. (Image 08 and 09) On solid media, the organism displayed the ability to produce a yellow pigment (Image 10) when cultured in both dark and light conditions at 37oC. However, this pigment was only produced in the light when culture was incubated at room temperature (25oC).
ANTIBIOTIC SUSCEPTIBILITY TESTING