FLOW CYTOMETRY:
Results: Cell suspension immunophenotypic studies were performed on the bone marrow aspirate and one region was analyzed.
Region 1 represents the small non-complex cells (52% of the events).
VIABILITY: 99%
Antigen | Usual Specificity | % Positive |
---|---|---|
Kappa + | B Cell Subset | 2 |
Lambda + | B Cell Subset | 3 |
Kappa:Lambda Ratio | 0.7 | |
CD19+ | B Cell | 11 |
CD19+/CD5+ | B Cell Subset | 3 |
CD20+ | B Cell | 6 |
CD10+ (Calla) | B Cell Subset | 3 |
Antigen | Usual Specificity | % Positive |
---|---|---|
CD2+ | T Cell | 84 |
CD3+ Total | T Cell | 78 |
CD5+/CD19- | T Cell | 74 |
CD7+ | T Cell | 72 |
CD4+/CD8- | T Helper | 13 |
CD8+/CD4- | C Cytotoxic/Suppressor | 63 |
CD4+/CD8+ | T Cell Subset | 1 |
CD4:CD8 Ratio | T Helper:Suppressor Ratio | 0.2 |
CD16,CD56+/CD3+ | T Cell Subset | 2 |
CD16,CD56+/CD3- | NK Cells | 7 |
Antigen | Usual Specificity | % Positive |
---|---|---|
CD15+ | Myeloid | 4 |
CD34+ | Stem Cell | 4 |
Antigen | Usual Specificity | % Positive |
---|---|---|
CD45+ | Leukocytes | 96 |
HLA DR+ | B, Myeloid, Activated T | 78 |
TdT+ | Lymphoblasts (Myeloblasts) | 2 |
Cyto CD3+ | T Cells | 76 |
CD22+ | 2 |
Interpretation:
Flow cytometric immunophenotypic studies reveal an increased population of CD3+ T-lymphocytes (78%). This poulation also showed a low CD4:CD8 ratio (0.2) and strong expression of HLA-DR antigen (78%). These findings are consistent with infectious mononucleosis.