Alexandra Collin de l'Hortet, PhD
Research Assistant Professor of Pathology


Dr. Collin de l'Hortet is a member of the Division of Experimental Pathology.

Office Location:
S420 BST
200 Lothrop Street
Pittsburgh, PA 15261
Contact Information:
Office Telephone: 412-624-3368
Fax: 412-648-1916
Email: Alexandra.collin@pitt.edu

Education

  • BSc, Magna Cum Laude, 2008 - Francois Rabelais University
  • MSc, Summa Cum Laude, 2010 - Paris Diderot University
  • PhD, Summa Cum Laude, 2014 - Paris Descartes University

Research Interests

Dr. Collin de l'Hortet is a Research Assistant Professor in the Department of Pathology at the University of Pittsburgh. She graduated Summa Cum Laude with an MSc in Genetics from Paris Diderot University in 2010, and was awarded a PhD degree with highest honors in Molecular Biology and Therapeutics from Paris Descartes University in 2014. Prior to undertaking her PhD, she worked as a pre-doc fellow at New York University where she built a genetic editing tool. That experience provided acute insight into molecular and cell biology, and deep expertise in stem cell biology and regenerative medicine. During her PhD, she received two doctoral grants from the French Ministry of Higher Education and Research and the French National League Against Cancer to work on the regeneration of fatty livers. She characterized the role of the endocrine system, particularly growth hormone, on fatty liver regeneration by controlling downstream molecular actors in the liver. Dr. Collin de l'Hortet joined the University of Pittsburgh in 2014 to pursue a postdoctoral fellowship where she used her multi-disciplinary training to develop cell and liver tissue engineering techniques to model human steatotic liver tissue with human iPS cells. She has successfully created human iPS cell lines carrying inducible Cas9 systems for high efficiency genetic engineering (currently filled for a PCT patent application), designed hepatic differentiation protocols, and generated mini human livers with iPS-derived human hepatocytes. She received a Philippe Foundation Award for this work in 2015 and 2016 and was the sole recipient of the Pathology Postdoctoral Research Training Program Grant from the University of Pittsburgh in 2016.

Dr. Collin de l'Hortet's research focuses on liver medicine, liver regeneration and liver diseases such as non-alcoholic liver diseases. She believes biomedical research needs to focus on human tissue to study and understand human diseases and her goal is to bring the potential of human cell and tissue engineering to understand clinically relevant liver mechanisms. To do so, Dr. Collin de l'Hortet is working on providing comprehensive tools for the study of human liver regenerative medicine and diseases that can lead to eventually treat liver diseases such as fibrosis, cirrhosis, metabolic diseases and hepatocellular carcinomas.

Selected Publications

  • Liver-enriched transcription factor expression relates to chronic hepatic failure in humans. Guzman-Lepe J, Cervantes-Alvarez E, Collin de l'Hortet A, Wang Y, Mars WM, Oda Y, Bekki Y, Shimokawa M, Wang H, Yoshizumi T, Maehara Y, Bell A, Fox IJ, Takeishi K, Soto-Gutierrez A. Hepatol Commun. 2018 Mar 23;2(5):582-594
  • Understanding Liver Regeneration: From Mechanisms to Regenerative Medicine. Gilgenkrantz H, Collin de l'Hortet A. Am J Pathol. 2018 Jun;188(6):1316-1327
  • SIRT1 Disruption in Human Fetal Hepatocytes Leads to Increased Accumulation of Glucose and Lipids. Tobita T, Guzman-Lepe J, Takeishi K, Nakao T, Wang Y, Meng F, Deng CX, Collin de l'Hortet A, Soto-Gutierrez A. PLoS One. 2016 Feb 18;11(2):e0149344.
  • Liver-Regenerative Transplantation: Regrow and Reset. Collin de l'Hortet A, Takeishi K, Guzman-Lepe J, Handa K, Matsubara K, Fukumitsu K, Dorko K, Presnell SC, Yagi H, Soto-Gutierrez A. Am J Transplant. 2016 Jun;16(6):1688-96
  • GH administration rescues fatty liver regeneration impairment by restoring GH/EGFR pathway deficiency. Collin de l'Hortet A, Zerrad-Saadi A, Prip-Buus C, Fauveau V, Helmy N, Ziol M, Vons C, Billot K, Baud V, Gilgenkrantz H, Guidotti JE. Endocrinology. 2014 Jul;155(7):2545-54
  • GH receptor plays a major role in liver regeneration through the control of EGFR and ERK1/2 activation. Zerrad-Saadi A, Lambert-Blot M, Mitchell C, Bretes H, Collin de l'Hortet A, Baud V, Chereau F, Sotiropoulos A, Kopchick JJ, Liao L, Xu J, Gilgenkrantz H, Guidotti JE. Endocrinology. 2011 Jul;152(7):2731-41