Clinical Flow Cytometry Laboratory - Test Specifications

  • Leukemia Panels
  • CLL/Lymphoma Panels
  • T Lymphocyte Panels

Deliver all samples directly to the Flow Cytometry Laboratory.

    Clinical Flow Cytometry Lab
    Clinical Laboratory Building
    3477 Euler Way
    Room 9032
    Pittsburgh, PA 15213

    Phone: (412) 864-6173
    Fax: (412) 864-6102

Flow Cytometry Lab Hours of Operation

Monday through Friday: 8:00 am to 6:00 pm. Emergency specimens can be processed on Saturday. The laboratory MUST be notified by 12 noon and the specimens must arrive by 1 pm.

For Saturday emergency specimens, please page the clinical pathology resident at 572-5851. The hematopathologist on call can be reached through the UPMC Oakland operator at 647-2345.

The lab is closed on Sunday and the following holidays: New Year’s Day, Martin Luther King Day, Memorial Day, July 4, Labor Day, Thanksgiving Day and Christmas.

Leukemia, CLL / Lymphoma Panel

Test Description

These tests utilize a panel of monoclonal antibodies in the immunophenotypic analysis of hematopoietic and lymphoid proliferation. The panels are used to assess cell lineage and to look for features that support a neoplastic rather than reactive proliferation.

Specimen Requirements

  • Bone marrow aspirate: Minimum of 2 ml in a heparinized (green top) tube.

  • Peripheral Blood: One EDTA (preferred; purple top) tube or heparinized (green top) tube with at least 5 ml of blood.    NOTE: Peripheral blood or bone marrow blast count or target population should be at least 25%. If bone marrow, send one non-heparinized, unstained bone marrow slide if possible.

  • Lymph nodes: Preferably 1 cm3 fresh tissue in RPMI or any other growth media. Normal saline may be used if RPMI is unavailable and transport time is kept to a minimum. A small portion of all tissues (or other tissues) will be processed for histologic sections.

  • Body fluids: Require a minimum of approximately 100,000 cells (e.g. 100 cells/μl x 1 ml; 10 cells/μl x 10 ml)

    Fine needle aspirates: Place cores (preferably two) and any additional aspirate in RPMI or other growth media.Normal saline may be used if RPMI is unavailable and transport time is kept to a minimum.

  • Special Instructions: In all cases, notify the lab before the specimen is sent (412-864-6173). If possible, notify lab one day in advance. Specimens must be received by 5:00 pm on Fridays, and by 3 pm the day before a holiday. If a late arrival is anticipated, please call the laboratory to confirm that the test can be performed. Send a COMPLETED requisition. Send all specimens at room temperature. Use adequate safety measures in transporting sample.

T-Lymphocyte Test Specifications

Test Description

This test may be identified as T Cell Subsets, T and B Cells, T4/T8 counts and other synonyms. The complete CDC recommended test panel includes a Total T or Pan T antibody, a Total B cell or Pan B antibody, a Total Helper antibody, a Total Suppressor antibody, the Helper/Suppressor, and a Total Natural Killer antibody. There are no functional tests associated with these antibodies.

A limited CD4 evaluation can be performed if requested. However, this is not endorsed by the CDC.

Specimen Requirements: One 5 ml EDTA (purple top) tube or two BD microtainer (purple top) pediatric tubes. Store sample at room temperature.

DNA By Flow Analysis

Test Description

This test may be identified as DNA ploidy, DNA/cell cycle analysis, and DNA analysis of cells. This test involves the examination of cellular DNA content. A DNA index is established for diploid and aneuploid cells in tumors, in addition to providing cell cycle statistics. This testing is sent to a reference laboratory.

Specimen Requirements

  • Whole blood: 10 ml yellow (ACD), purple (EDTA) or green (heparin) anticoagulated venous blood kept at ambient temperature and analyzed within 24 hours of drawing.

  • Bone marrow: 1 ml heparinized marrow kept at ambient temperature and analyzed within 24 hours of drawing.

Division of Hematopathology Contacts

  • Sara A. Monaghan, MD - Director, Clinical Flow Cytometry Laboratory, 412-647-8504
  • Wendy Shallenberger - Lead Technologist, Flow Cytometry, 412-647-6518
  • Steven H. Swerdlow, MD - Director, Division of Hematopathology, 412-647-5191