Pathology Graduate Training Program
Cellular and Molecular Pathology (CMP) -

Name: Dan Wang

Undergraduate Degree: B.S.
Undergraduate Degree Year: 2003
Undergraduate Institution: Zhejiang University
Undergraduate Major: Biotechnology

Academic Status: 2nd Year

Graduate Program: Experimental Pathology

Thesis Advisor: Youhua Liu, Ph.D.

Thesis Title/Research Topic: Characterization of the Interaction of the Ternary Complex, Integrin-linked kinase (ILK), Nephrin and NCK

Current Research Description: Nephirn, as a podocyte protein, plays important role in establishing the size-selective filtration barrier of the kidney. Previous studies in our lab have already shown that podocyte specific ablation of ILK resulted in the aberrant distribution of nephrin and function deletion of foot process, also, adaptor protein NCK is involved in the formation of ILK/nephrin complex in podocyte in vivo. We constructed various truncated and mutated versions of ILK, nephrin and NCK to define the interaction of this ternary complex. The yeast hybridization will be used to confirm the direct association of two proteins and find the new binding proteins. Immunofluorescence staining, immunoprecipitation, GST pull down assay are used to specify the binding domain within the mutations. The characterization of interaction of the complex may provide novel insights into the pathogenesis of podocyte dysfunction and proteinuria in many common glomerular diseases.

Undergraduate Honors: 2000 Sep, 2001 Sep, 2002 Sep, Scholarship for Excellent Holder; 2003 Jun, Honored Undergraduate of Zhejiang University