IMMUNOHISTOCHEMICAL FINDINGS:
A panel of immunohistochemical stains was performed to better characterize the atypical follicular structures and the atypical focal spindle cell proliferation. The following results were found:
Antigen/Antibody | Usual Reactivity | Result |
---|---|---|
anti-kappa | B cell Subset | Scattered positive plasma cells. |
anti-lambda | B cell Subset | Scattered positive plasma cells. |
CD20/L26 (Images A & B) |
B cell | Numerous positive cells in follicular structures, and many positive cells in interfollicular areas. |
CD21 | Follic, Dendritic cells | Highlights follicles and shows positivity in focal atypical spindle cell areas. |
bcl-2 | lymph subset, other | Positive in abnormal follicular structures. |
CD10 | B cell subset | Some abnormal follicles strongly positive, some with weaker positivity; interfollicular areas have scattered positive cells. |
CD3 | T cells | Moderate number of scattered cells in the interfollicular regions in the part of the node with abnormal follicles, larger numbers in the interfollicular areas of normal part of node. |