Thomas Lozito, PhD
Assistant Professor

Dr. Thomas Lozito
Dr. Lozito is a member of the Department of Orthopaedic Surgery.

Office Location:
450 Technology Drive
Bridgeside Point II Building
Room 217

Contact Information:
Office Telephone: 732-678-6131
Email: tpl9@pitt.edu

Education

  • BS - Johns Hopkins University
  • PhD - University of Cambridge

Research Interests

The Lozito laboratory studies limb and tail regeneration in salamanders and lizards with the long-term goal of developing strategies for improving mammalian regeneration. I am particularly interested in what accounts for divergent regenerative capabilities among vertebrates, and my lab at the University of Pittsburgh is one of the only labs in the world that studies both lizard and salamander tissue regeneration. For example, lizards and salamanders both exhibit the amazing ability to regenerate amputated tails. However, while the salamander regenerated tail is a perfect copy of the original, the lizard regenerates an "imperfect replicate". The most striking of these "imperfections" concerns the skeleton, which takes the form of an unsegmented cartilage tube rather than a vertebral column. My research compares skeletal regeneration in lizards (Lepidodactylus lugubris) and salamanders (Ambystoma mexicanum), looking for what they have in common, how they differ, and why they end with such different regenerative outcomes. For example, we have determined that lizard and salamander blastemas (the collection of cells that differentiate into the tissue of the regenerated tail) form in very different ways, findings with potential for improving mammalian regeneration. regulates HNF4 in fibrosis are currently being investigated in rodent models of fibrosis and carcinogenesis.

Selected Publications

  • Londono R, Wenzhong W, Wang B, Tuan RS, Lozito TP. Cartilage cell fate and origins during lizard tail regeneration. Front Bioeng Biotechnol. 2017 In Press.
  • Lozito TP, Tuan RS. Lizard Tail Regeneration Combines Aspects of Fracture Healing and Blastema-Based Regeneration. Development. 2016 Aug;143(16):2946-57.
  • Lozito TP, Tuan RS. Lizard Tail Regeneration As An Instructive Model of Enhanced Healing Capabilities In An Adult Amniote. Connect Tissue Res. 2016 Jul 26:1-10
  • Lozito TP, Tuan RS. Lizard Tail Regeneration: Regulation of Two Distinct Cartilage Regions by Indian Hedgehog. Dev Biol. 2015 Mar;399(2):249-62.
  • Lozito TP, Tuan RS. Endothelial and cancer cells interact with mesenchymal stem cells via both microparticles and secreted factors. J. Cell Mol Med. 2014 Dec;18(12):2372-84.
  • Lozito TP*, Lin H*, Alexander PG, Gottardi R, Tuan RS (*Equal Author Contribution). Stem cell-based microphysiological osteochondral system to model tissue response to interleukin-1?. Mol. Pharmaceutics. 2014 Jul;11(7):2203-12.
  • Alexander PG, Gottardi R, Lin H, Lozito TP, Tuan RS. Three-dimensional osteogenic and chondrogenic systems to model osteochondral physiology and degenerative joint diseases. Exp Biol Med. 2014 Sep;239(9):1085-95.
  • Lozito TP, Jackson WM, Nesti LJ, Tuan RS. Human mesenchymal stem cells generate a distinct pericellular zone of MMP activities via binding of MMPs and secretion of high levels of TIMPs. Matrix Biology. 2014 Feb;34:132-43.
  • Cui C, Chatterjee B, Lozito TP, Zhang Z, Francis R.J, Yagi H, Swanhart LM, Sanker S, Francis D, Yu Q, San Agustin JT, Puligilla C, Chatterjee T, Tansey T, Liu X, Kelley MW, Spiliotis ET, Kwiatkowski AV,
  • Tuan RS, Pazour GJ, Hukriede NA, Lo CW. Wdpcp, a PCP Protein Required for Ciliogenesis, Regulates Directional Cell Migration and Cell Polarity by Direct Modulation of the Actin Cytoskeleton. Plos Biology. 2013 Nov;11(11).
  • Lozito TP, Tuan RS. Endothelial cell microparticles act as centers of MMP-2 activation and for vascular matrix remodeling. J Cell Physiol. 2012 Feb;227(2):534-49.
  • Jackson WM, Lozito TP, Djouad F, Kuhn NZ, Nesti LJ, Tuan RS. 2011. Differentiation and regeneration potential of mesenchymal progenitor cells derived from traumatized muscle tissue. J Cell Mol Med. 2011 Nov;15(11):2377-88.
  • Lozito TP, Tuan RS. Mesenchymal stem cells inhibit both endogenous and exogenous MMPs via secreted TIMPs. J Cell Physiol. 2011 Feb;226(2):385-96.
  • Lozito TP, Kuo CK, Taboas JM, Tuan RS. Human mesenchymal stem cells express vascular cell phenotypes upon interactions with endothelial cell matrix. J Cell Biochem. 2009 Jul;107(4):714-22.
  • Lozito TP, Taboas JM, Kuo CK, Tuan RS. Mesenchymal stem cell modification of endothelial cell matrix regulates their vascular differentiation. J Cell Biochem. 2009 Jul;107(4):706-13.