Kumiko Isse, MD, PhD
Research Instructor

Dr. Isse is a member of the Division of Transplant Pathology.

Office Location:
BST E1507
200 Lothrop Street
Pittsburgh, PA 15213
Contact Information:
Lab Telephone: 412-624-1304
Email: issek@upmc.edu


  • MD - Kanazawa University, Japan, 2001
  • PhD - Kanazawa University, Japan, 2005

Research Interests

Dr. Isse is interested in liver immunology, especially microenvironment around bile duct. Dr. Isse is leading the technical developments and trouble shooting for the multiplex quantum dot immunohistochemistry combined with whole digital slide imaging and automated image analysis. This effort requires tremendous dedication to detail and development of staining protocols, customized software development for capturing images, and uniquely modifying our digital scanning system to enable this project. We can now stain up to 5 different antigens and a single tissue section, followed by WSI creation and analysis of any channel (-s) alone or in any combination. This pioneering technology will greatly enhance the capabilities of the project.


  • Medical Doctor / Japan, 2001
  • Autopsy for Human / Japan, 2005


Human general surgical/anatomical pathology, liver pathology, multiplex immunostaining, image analysis

Awards and Honors

  • 1999 - Encouragement prize of Liver Cancer Study Group of Japan
  • 2001 - Encouragement prize from a president of Kanazawa University
  • 2003 - Scholarship Fund from Japan Society for the Promotion of Science
  • 2004 - Awarded the Honjin Foundation
  • 2009 - Poster session in Thomas Starzl Transplant Institute Fall Retreat

Selected Publications

View Dr. Isse's publications on PubMed

  1. Isse K, Lesniak A, Grama K, Roysam B, Minervini MI, Demetris AJ.: Digital Transplantation Pathology: Combining Whole Slide Imaging, Multiplex Staining and Automated Image Analysis.Am J Transplant. 2012, Jan: 12(1):27-37.
  2. Lunz J, Ruppert KM, Cajaiba MM, Isse K, Bentlejewski CA, Minervini M, Nalesnik MA, Randhawa P, Rubin E, Sasatomi E, Vera ME, Fontes P, Humar A, Zeevi A, Demetris AJ.: Re-examination of the Lymphocytotoxic Crossmatch in Liver Transplantation: Can C4d Stains Help in Monitoring? Am J Transplant. 2011, Jan:12(1):171-82
  3. Turnquist HR, Zhao Z, Rosborough BR, Liu Q, Castellaneta A, Isse K, Wang Z, Lang M, Stolz DB, Zheng XX, Demetris AJ, Liew FY, Wood KJ, Thomson AW.: IL-33 expands suppressive CD11b+ Gr-1 int and regulatory T cells, including ST2L+ Foxp3+ cells, and mediates regulatory T cell-dependent promotion of cardiac allograft survival. J Immunol. 2011 Nov 1;187(9):4598-610. Epub 2011 Sep 26.
  4. Hara H, Koike N, Long C, Piluek J, Roh DS, SundarRaj N, Funderburgh JL, Mizuguchi Y, Isse K, Phelps CJ, Ball SF, Ayares DL, Cooper DK.: Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs. Invest Ophthalmol Vis Sci. 2011 Jul 15;52(8):5278-86. Print 2011 Jul.
  5. Ueki S, Castellaneta A, Yoshida O, Ozaki K, Zhang M, Kimura S, Isse K, Ross M, Shao L, Stolz DB, Thomson AW, Demetris AJ, Geller DA, Murase N.: Hepatic B7-H1 expression is essential to control cold ischemia/reperfusion injury after mouse liver transplantation. Hepatology. 2011 July:54(1):216-228
  6. Isse K, Grama K, Abbott IM, Lesniak A, Lunz JG, Lee WM, Specht S, Corbitt N, Mizuguchi Y, Roysam B, Demetris AJ: Adding Value to Liver (and Allograft) Biopsy Evaluation Using a Combination of Multiplex Quantum Dot Immunostaining, High-Resolution Whole-Slide Digital Imaging, and Automated Image Analysis. Clin Liver Dis.2010 Nov;14(4):669-85
  7. Isse K, Specht SM, Lunz JG 3rd, Kang LI, Mizuguchi Y, Demetris AJ: Estrogen stimulates female biliary epithelial cell interleukin-6 expression in mice and humans. Hepatology. 2010 Mar;51(3):869-80.
  8. Toso C, Isse K, Demetris AJ, Dinyari P, Koh A, Imes S, Kin T, Emamaullee J, Senior P, Shapiro AM: Histologic graft assessment after clinical islet transplantation. Transplantation. 2009 Dec 15;88(11):1286-93.
  9. Li G, Han C, Xu L, Lim K, Isse K, Wu T: Cyclooxygenase-2 prevents fas-induced liver injury through up-regulation of epidermal growth factor receptor. Hepatology. 2009 Sep;50(3):834-43.
  10. Harada K, Shimoda S, Sato Y, Isse K, Ikeda H, Nakanuma Y: Periductal interleukin-17 production in association with biliary innate immunity contributes to the pathogenesis of cholangiopathy in primary biliary cirrhosis. Clin Exp Immunol. 2009 Aug;157(2):261-70.
  11. Harada K, Sato Y, Ikeda H, Isse K, Ozaki S, Enomae M, Ohama K, Katayanagi K, Kurumaya H, Matsui A, Nakanuma Y: Epithelial-mesenchymal transition induced by biliary innate immunity contributes to the sclerosing cholangiopathy of biliary atresia. J Pathol. 2009 Apr;217(5):654-64.