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Research Interests:
Keywords: Encephalitis, Viral infection, Macrophages / microglia, Innate immune response, Inflammation
Dr. Wiley's research concentrates on the pathogenesis of neurodegenerative diseases and in particular on viral and age induced nervous system diseases. Viruses damage the nervous system either by direct infection of neural cells or by secondary effects of an immune response. In recent years his studies have focused on central nervous system influenza and retroviral infections and have pioneered the use of molecular and imaging techniques to quantitatively assess viral burden. This same technology is now being applied to quantify neurological damage and the immune / neuroinflammatory response with specific attention focused on disruption of the extracellular matrix and how this leads to synaptic damage.
Trainees in Dr. Wiley's laboratory have the opportunity to learn a wide variety of techniques related to studying degeneration of the nervous system. Both whole organism and tissue culture techniques are available using histological techniques that include; immunocytochemistry, in situ hybridization and ultrastructural analysis. Training on laser confocal and electron microscopes is available. Protein techniques include; antibody purification and tagging. Molecular techniques include; RNA and DNA extraction from tissues, competitive PCR probe construction and quantitation. Lab rotation projects involve quantitation of mRNA and protein expression in rodent, human and non-human primate nervous system tissue in vivo or in vitro.
Selected Publications:
View Dr. Wiley's publications on PubMed.
Rowe T, Leon AJ, Crevar CJ, Carter DM, Xu L, Ran L, Fang Y, Cameron CM, Cameron MJ, Banner D, Ng DCK, Ran R, Weirback HK, Wiley CA, Kelvin DJ, Ross TM: Modeling host responses in ferrets during A/California/07/2009 influenza infection. Virology 401(2): 257-65, 2010. PMCID: PMC2862141
Bonneh-Barkay D, Zagadailov P, Zou H, Niyonkuru C, Figley M, Starkey A, Wang G, Bissel SJ, Wiley, CA and Wagner A. YKL-40 expression in traumatic brain injury - an initial analysis. J Neurotrauma 27:1-9, 2010. PMCID: PMC2942903
Kofler J and Wiley CA; Microglia: Key innate immune cells of the brain. Toxicologic Pathology 39:103-114, 2011. PMID: 21078923
Wiley CA, Carter DM, Ross TM, Bissel SJ; Absence of fetal transmission of H1N1 despite severe maternal infection. Influenza and other Respiratory Viruses 2011 Nov 22. doi: 10.1111/j.1750-2659.2011.00310.x. [Epub ahead of print] PMID:22103260
Bonneh-Barkay D, Bissel SJ, Kofler J, Starkey A, Wang G, Wiley, CA; Astrocyte and macrophage regulation of YKL-40 expression and cellular response in neuroinflammation. Brain Pathology 2011 doi: 10.1111/j.1750-3639.2011.00550.x
Bissel SJ, Giles BM, Wang G, Olevian DC, Ross TM, Wiley CA; Acute Murine H5N1 Influenza A Encephalitis. Brain Pathology , 201110.1111/j.1750-3639.2011.00514.x
Giles BM, Bissel SJ, Craigo JK, Dealmeida DR, Wiley CA, Tumpey TM, Ross TM; Elicitation of Anti-1918 Influenza Virus Immunity Early in Life Prevents Morbidity and Lower Levels of Lung Infection by 2009 Pandemic H1N1 Influenza Virus in Aged Mice. J Virol. 2011 Nov 30. [Epub ahead of print] PMID:22130546 [PubMed - as supplied by publisher]
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