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Research Interests:
Dr. Wadowsky is exploring the use of molecular techniques for diagnosis of infections in children. He has recently developed a real time RT PCR assay with a degenerate TaqMan MGB probe for detecting rhinovirus in respiratory tract specimens. He has published studies on development and evaluation of PCR-based assays for the quantitation of Epstein-Barr Virus DNA load in blood specimens and for the detection of Bordetella pertussis, Mycoplasma pneumoniae, and Chlamydia pneumoniae in respiratory tract specimens. Dr. Wadowsky is also interested in utilizing loop-mediated isothermal amplification technology for developing point-of-care testing assays for detection a variety of infectious agents including B. pertussis, methicillin resistant Staphylococcus aureus, influenza virus, and respiratory syncytial virus. Dr. Wadowsky is an authority on Legionnaires' disease and Aspergillus infections in hospitalized patients.
Dr. Wadowsky is Chief of Microbiology and Director of Pediatric Molecular Microbiology at the Children's Hospital of Pittsburgh.
Selected Publications:
View Dr. Wadowsky's publications on PubMed.
Michelson P, Watkins B, Webber SA, Wadowsky RM, Michaels MG. 2008. Screening for PTLD in lung and heart-lung transplant recipients by measuring EBV DNA load in bronchoalveolar lavage fluid using real time PCR. Pediatr Transplant 12:464-468.
Wadowsky RM, Laus S, Green M, Webber SA, Rowe D. Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR. J Clin Microbiol. 2003 Nov;41(11):5245-9.
Pitetti RD, Laus S, Wadowsky RM. Clinical evaluation of a quantitative real time polymerase chain reaction assay for diagnosis of primary Epstein-Barr virus infection in children. Pediatr Infect Dis J. 2003 Aug;22(8):736-9.
Wadowsky RM, Castilla EA, Laus S, Kozy A, Atchison RW, Kingsley LA, Ward JI, Greenberg DP. Evaluation of Chlamydia pneumoniae and Mycoplasma pneumoniae as etiologic agents of persistent cough in adolescents and adults. J Clin Microbiol. 2002 Feb;40(2):637-40.
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