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Research Interests:
Dr. Soto-Gutierrez's research is focused on the development of new technologies for organ replacement using regenerative medicine approaches (bioengineering, cell transplantation and organ engineering) to generating entire replacement organs, Dr. Soto-Gutierrez's laboratory use the structural connective tissue of discarded livers as a scaffold for growing new liver tissue for transplantation and primary liver isolated cells. His laboratory is actively working in liver cell differentiation and understanding maturation of ES or iPS cells using interactions with liver non-parenchymal liver cells, 3D liver matrix and different molecules. While portions of the studies are carried out in organ culture systems, several ongoing studies are performed in special animal models of liver regeneration to understand hepatic tissue assembling and regeneration using auxiliary partial orthotopic liver transplantation (APOLT).
In addition, Dr. Soto-Gutierrez's laboratory is also interested in strategies for liver repopulation and the study of liver regeneration in disease state (e.g. acute or chronic liver failure). To this end, several lines of collaboration are open with the Center for Innovative Regenerative Therapies (CIRT) and different laboratories in the Department of Pathology and the STI.
Selected Publications:
Soto-Gutierrez A, Zhang L, Medberry CJ, Fukumitsu K, Faulk D, Jiang H, Reing JE, Gramignoli R, Komori J, Ross M, Nagaya M, Lagasse E, Beer Stolz D, Strom S, Fox IJ, Badylak SF. A Whole Organ Regenerative Medicine Approach for Liver Replacement. Tissue Eng Part C Methods. 2011 Jun;17(6):677-86.
Uygun B, Soto-Gutierrez A, Yagi H, Izamis ML, Shulman C, Milwid J, Kobayashi N, Nahmias Y, Tilles A, Berthiaume F, Hertl M, Yarmush ML, Uygun K. Organ Re-Engineering: Development of a Transplantable Recellularized Liver Graft using Decellularized Liver Matrix. Nat Medicine. 2010 2010 Jul;16(7):814-20.
Navarro-Alvarez N, Soto-Gutierrez A, Chen Y, Caballero-Corbalan J, Hassan W, Takei H, Iwamuro M, Yamamoto H, Tanaka N, Fox IJ and Kobayashi N. Intramuscular Transplantation of Engineered Hepatic Tissue Constructs Corrects Acute and Chronic Liver Failure in Mice. J Hepatol. 2010;52(2):211-9.
Soto-Gutierrez A, Navarro-Alvarez N, Yagi H, Nahmias Y, Yarmush ML, Kobayashi N, Engineering of an Hepatic Organoide to Develop Liver Assist Devices. Cell Transplant. 2010, 19(6):815-22.
Basma H, Soto-Gutie?rrez A, Govardhana Rao Yannam1, LiuL, Ito R, Yamamoto T, Ellis E, Carson SD, Sato S, Chen Y, Muirhead D, Navarro-A?lvarez N, Wong R, Roy-Chowdhury J, Platt JL, Mercer DF, Miller JD, Strom SC, Kobayashi N, Fox IJ. Differentiation and Transplantation of Human Embryonic Stem Cell-Derived Hepatocytes. Gastroenterology. 2009;136(3):990-9.
Soto-Gutiérrez A, Navarro-Alvarez N, Zhao D, Rivas-Carrillo JD, Lebkowski J, Tanaka N, Fox IJ, Kobayashi N. Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Nat Protoc. 2007;2(2):347-56.
Soto-Gutiérrez A, Kobayashi N, Rivas-Carrillo JD, Navarro-Alvarez N, Zhao D, Okitsu T, Noguchi H, Basma H, Tabata Y, Chen Y, Tanaka K, Narushima M, Miki A, Ueda T, Jun HS, Yoon JW, Lebkowski J, Tanaka N, Fox IJ. Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes. Nat Biotechnol. 2006;24(11):1412-9.
Reviews
Fukumitsu K, Yagi H, Soto-Gutierrez A. Bioengineering in Organ Transplantation: Targeting the Liver. Transplant Proc. 2011 (In press)
Soto-Gutierrez A, Tafaleng E, Kelly V, Roy-Chowdhury J, Fox IJ. Human Liver Diseases Modeling and Therapy using Induced Pluripotent Stem Cells: Are We There Yet?. Hepatology; 2011;53(2):708-11.
Soltys KA, Soto-Gutiérrez A, Nagaya M, Baskin KM, Deutsch M, Ito R, Shneider BL, Squires R, Vockley J, Guha C, Roy-Chowdhury J, Strom SC, Platt JL, Fox IJ. Barriers to the successful treatment of liver disease by hepatocyte transplantation. J Hepatol. 2010 Oct;53(4):769-74.
Navarro-Alvarez N, Soto-Gutierrez A, Kobayashi N. Hepatic stem cells and liver development. Methods Mol Biol. 2010;640:181-236.
Soto-Gutierrez A, Yagi H, Uygun BE, Navarro-Alvarez N, Uygun K, Kobayashi N, Yang YG and Yarmush ML. Cell Delivery: From Cell Transplantation to Organ Engineering. Cell Transplant. 2010, 19(6):655-65.
Yagi H, Tafaleng E, Nagaya M, Strom SC, Fox IJ, Soto-Gutierrez A. Embryonic and Induced Pluripotent Stem Cells as a Model for Liver Disease. Crit Rev Biomed Eng. 2009;37(4-5):377-98.
Soto-Gutierrez A, Navarro-Alvarez N, Yagi H, Yarmush ML. Stem Cells for Liver Repopulation. Curr Opin Org Transplant. 2009 Dec;14(6):667-73.
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