Hun-Way Hwang, M.D., Ph.D.
Assistant Professor of Pathology
Dr. Hwang is Assistant Professor in the Section of Laboratory Medicine and Division of Experimental Pathology
S754 Scaife Hall
Pittsburgh, PA 15261
Office Telephone: 412-648-7300
How different types of cells in intact tissues regulate their mRNAs in physiologic and disease conditions remains largely unexplored. My research goal is to study mRNA regulation in both physiologic and disease scenarios with a special emphasis on 3-prime UTR-mediated regulation. Cells use 3-prime untranslated regions (3-prime UTRs) as dynamic platforms to regulate mRNA stability, localization and translational efficiency in response to outside stimuli by interacting with regulators such as microRNAs (miRNAs) and RNA-binding proteins (RBPs). Moreover, erroneous 3-prime UTR-mediated mRNA regulation is frequently involved in human disease. One important mechanism to generate mRNA isoforms with distinct 3-prime UTR sequences is through alternative polyadenylation (APA), which has been recently shown to play important roles in fundamental cellular processes including proliferation and differentiation, and also in human disease such as cancer, vascular thrombosis, and neurological disorders. During my postdoctoral studies, I developed new tools to generate APA maps and mRNA expression profiles from specific cell types in intact tissues. These tools provide an exciting opportunity to explore new biology that is not feasible with existing methods. Combining high-throughput sequencing, mouse genetics and molecular biology techniques, my laboratory will apply these tools to discover and to study important APA events in human biology and disease.
Selected PublicationsView Dr. Hwang's publications on PubMed
- Hwang HW, Park CY, Goodarzi H, Fak JJ, Mele A, Moore MJ, Saito Y, Darnell RB. PAPERCLIP identifies microRNA targets and a role of CstF64/64tau in promoting non-canonical poly(A) site usage. Cell Reports. 2016 Apr 12;15(2):423-35.
- Hwang HW, Baxter LL, Loftus SK, Cronin JC, Trivedi NS, Borate B, Pavan WJ. Distinct microRNA expression signatures are associated with melanoma subtypes and are regulated by HIF1A. Pigment Cell Melanoma Res. 2014 Sep;27(5):777-87.
- Hwang HW, Wentzel EA, Mendell JT. Cell-cell contact globally activates microRNA biogenesis. PNAS. 2009 Apr 28;106(17):7016-21.
- Hwang HW, Wentzel EA, Mendell JT. A hexanucleotide element directs microRNA nuclear import. Science. 2007 Jan 5;315(5808):97-100.
- Hwang HW, Mendell JT. MicroRNAs in cell proliferation, cell death, and tumorigenesis. Br J Cancer. 2006 Mar 27;94(6):776-80.