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Research Interests:
Dr. Amoscato's research interests include mass spectrometry of peptides, proteins, lipid and carbohydrates; alterations in cellular lipids during apoptosis and mechanisms of apoptosis; sequencing of MHC peptide epitopes; matrix metalloproteases of dendritic cells; research in the area of biochemistry/immunology, especially as it applies to the interaction of various lymphokines and immunomodulators with cells of the immune system; the role of cell surface enzymes of the immune system; detection, isolation, purification and characterization of cell surface enzymes, antigens and novel cytokines; mechanism of NK cytolytic function; structure and function of NK cell-associated granule enzymes; surface aminopeptidases and endopeptidases of NK and dendritic cells; design and function of novel enzyme inhibitors; psychoneuroimmunology and the regulation of immune function by enkephalins and endorphins.
Selected Publications:
Thomas, R.L., C. M. Matsko, M. Lotze and A.A. Amoscato. (1999). Mass spectrometric identification of increased C16 ceramide levels during apoptosis. J. Biol. Chem.274:30580
Herr, W., E. Ranieri, A. Gambotto, L. Salvucci Kierstead, A. A. Amoscato, L. Gesualdo and W. J. Storkus (1999). CD8+ T-cell response to naturally-processed or synthetic EBV peptide epitopes in vitro: implications for peptide-based vaccination trials. Proc. Natl. Acad. Sci. 96:12033-8.
Dong, X., B. An, L. S. Salvucci Kierstead, W. J. Storkus, A. A. Amoscato, and R. D. Salter (2000). Modification of the N terminus of a class II epitope confers resistance to degradation by dendritic cells and enhances presentation to T cells. J. Immunol. 164:129-35.
Leite, J. F., A. A. Amoscato, and M. Cascio. (2000). Coupled proteolytic and mass spectrometry studies indicate novel topology for the glycine receptor. J. Biol. Chem. 275:13683-89.
Johnson, D. E., Gastman, B. R., Wieckowski, E., Wang, G-Q., Amoscato, A. A., Delach, S. M. and Rabinowich, H. 2000. Inhibitor of apoptosis protein hILP undergoes caspase-mediated cleavage during T-lymphocyte apoptosis. Cancer Res. 60: 1818-23.
Lee, Y. L., Chen, J. C., Amoscato, A. A., Bennouna, J., Spitz, D. R., Suntharalingam, M. and Rhee, J.G. 2001. Protective role of Bcl-2 in metabolic oxidative stress-induced cell death. J. Cell Science. 114 (Pt 4): 677-84.
Kao, H., A. A. Amoscato, P. Ciborowski and O. J. Finn. 2001. A new strategy for tumor antigen discovery based on in vitro priming of naïve T cells with dendritic cells. Clinical Cancer Research. 7 (3 Suppl): 773s-780s.
Kanto, T. M., P. Kalinski, O. Hunter, M. T. Lotze and A. A. Amoscato. 2001. Ceramide mediates tumor-induced dendritic cell apoptosis. J. Immunol. 167: 3773-84.
Matsko, C. M., Hunter, O., Rabionowich, H., Lotze, M. T. and Amoscato, A. A., 2001 Alterations in mitochondrial lipids during Fas- and radiation-induced apoptosis. Biochem. Biophys. Res. Commun. 287:1112-20.
Nam, S. Y., A. A. Amoscato and Y. J. Lee. 2002. Low glucose-enhanced TRAIL cytotoxicity is possibly mediated through the ceramide-Akt-FLIP pathway. Oncogene 21:337-346.
Ostrander, D. B., G. C. Sparanga, A. A. Amoscato, J. B. McMillin and W. Dowhan. 2001. Decreased cardiolipin synthesis corresponds with cytochrome c release in palmitate-induced cardiomyocyte apoptosis. J. Biol. Chem. 276: 38061-67.
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