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Research Interests:
My general research interests are: (1) to study mechanism of antigen uptake, processing and presentation by antigen presenting cells, especially dendritic cells; (2) to understand interaction between innate and adaptive immunity, particularly the role of lectin-like receptors, such as mannose receptor.
Currently, I am working with human agalactosyl IgG. Rheumatoid arthritis and some other autoimmune disease patients contain high levels of this IgG. We have shown that altered glycan on IgG can be recognized by mannose receptor on human dendritic cells and internalized into endosome. And this form of IgG is more accessible for processing and can be presented better to T cells. The mechanism is under investigation. In addition, we are applying HLA-DR4/IgG peptide complex tetramers to analyze specific T cells in the patients. We are hoping to have some insights into those autoimmune disease pathogenesis.
Carbohydrate-binding lectins on macrophages and dendritic cells, such as mannose receptor, can recognize some pathogenic microorganisms. They initiate signals for releasing inflammatory cytokines and some chemokines, as well as take up pathogens for clearing/processing and presentation.
We want apply the techniques and knowledge to design better vaccines and effective immuno-therapeutic protocols for cancer and infectious diseases
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Grants & Awards
- 2000 AAI-Huang Foundation Trainee Achievement Award, AAI (American Association of Immunologists), Bethesda, MD
- 1993-96 Dr.-M.-Scheel-Stiftung fuer Krebsforschung from Deutsche Krebshilfe (German Cancer Foundation), Bonn, Germany
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Publications:
- X. Dong, W.M. Amselgruber, H. Kaltner, H.-J. Gabius and F. Sinowatz. Affinity-purified antibodies against alpha-galactosyl residues from human serum: comparison of their binding in bovine testicular tissue with that of the Griffonia simplicifolia lectin (GSI-B4) and impact of labeling on epitope localization. European Journal of Cell Biology 1995; 68(1):96-101
- H.C. Siebert, C.W. von der Lieth, X. Dong, G. Reuter, R. Schauer, H.-J. Gabius and J.F. Vliegenthart. Molecular dynamics-derived conformation and intramolecular interaction analysis of the N-acetyl-9-O-acetylneuraminic acid-containing ganglioside GD1a and NMR-based analysis of its binding to a human polyclonal immunoglobulin G fraction with selectivity for O-acetylated sialic acids. Glycobiology 1996; 6(6):561-572
- D. Gupta, H. Kaltner, X. Dong, H.-J. Gabius and C.F. Brewer CF. Comparative cross-linking activities of lactose-specific plant and animal lectins and a natural lactose-binding immunoglobulin G fraction from human serum with asialofetuin. Glycobiology 1996; 6(8):843-849
- X. Dong, S. Andre, B. Hofer, K. Kayser and H.-J. Gabius. Disease type-associated increases of the plasma levels and ligand expression for natural alpha- or beta-galactoside-binding immunoglobulin G subfractions in patients with lung cancer. International Journal of Oncology 1997; 10(4):709-719
- M. Feili-Hariri, X. Dong, S.M. Alber, S.C. Watkins, R.D. Salter and P.A. Morel. Immunotherapy of NOD mice with bone marrow-derived dendritic cells. Diabetes 1999, 48(12):2300-2308
- X. Dong, W.J. Storkus and R.D. Salter. Binding and uptake of agalactosyl immunoglobulin G by mannose receptor on macrophages and dendritic cells. Journal of Immunology 1999, 163(10):5427-5434
- X. Dong, B. An, L.S. Kierstead, W.J. Storkus, A.A. Amoscato and R.D. Salter.
Modification of the N terminus of a class II epitope confers resistance to degradation by CD13 on dendritic cells and enhances presentation to T cells. Journal of Immunology 2000, 164(1):129-135
- S. Jin, M.J. Antinore, F.T. Lung, X. Dong, H. Chao, F. Fan, A.B. Colchagie, P. Blanck, P.P. Roller, A.J. Fornace Jr. and Q Zhan. The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression. Journal of Biological Chemistry 2000, 275(22):16602-16608
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