Department of Pathology

Division of Molecular Diagnostics

General Laboratory Topics Checklist

Rotating Fellow Name:________________________________

Rotation Period:______________________________________

	Check Status	Staff	Date
1. Sample receipt/ordering
2. Sample Handling
3. Send-Out Testing (N/A for hemepath fellows)
4. MDX Database
5. MDX Administration Licensing Billing Reimbursement
6. MDX Safety
7. MDX QC/QA/QI

Fellow signature: __________________________________ Date _______________

Department of Pathology

Division of Molecular Diagnostics

Fellow Name:________________________________

Rotation Period:______________________________________

	Check Status	Staff	Date
Southern Blot (e.g. IgH, TcR-beta, Fragile X)
1. Sample Preparation
2. Restriction Enzyme Digestion
3. Electrophoresis and Blotting
4. Probe Preparation
5. Hybridization
6. Exposure and Development
PCR analysis followed by agarose/PAGE sizing (IgH, TCR-gamma PCR)
1. Sample Preparation
2. PCR Set-up
3. Running PCR and Gel Electrophoresis
4. Detection of PCR Products
RT-PCR analysis qualitative (e.g. bcr-abl for CML, ALL or PML-RARalpha)
1. Sample Preparation
2. PCR Set-up
3. Running PCR and Gel Electrophoresis
4. Detection of PCR Products
Quantitative PCR (e.g. bcr-abl for CML – in development 3/2002)
1. Sample Preparation
2. RT-PCR Set-Up
3. Colorimetric Assay
4. Data Analysis

Fellow Signature: __________________ Date: ___________________________

UNIVERSITY OF PITTSBURGH CLINICAL CYTOGENETICS

TRAINING CHECKLIST FOR HEMATOPATHOLOGY FELLOW

Activity	Date Completed
Lab tour
Slide presentation
Review of recent literature to familiarize self with the fields of genetics, cytogenetics, cytogenetic methods, and applications to clinical medicine, especially with regards to hematology/oncology/ bone marrow transplantation
Read laboratory procedure manual in order to understand the rationale behind the methodologies and safety precautions used in the cytogenetic laboratory
Observe basic laboratory procedures
· cell culture initiation
· cell culture harvest
· banding
· chromosome analysis
· karyotyping
· Cytovision
· FISH
Practice karyotyping and interpret results in the form of cytogenetic reports
Scan G-banded slides to compare the results of PHA-stimulated peripheral blood, unstimulated peripheral blood, and bone marrow cultures to appreciate the different mitotic indices and appearance of chromosomes prepared under different culture conditions
Review files of interesting cases to observe the longitudinal changes in karyotypes over the course of treatment and to see how various cytogenetic findings are interpreted
Review files and literature of interesting cases with particular emphasis on commonly found hematopoietic chromosomal abnormalities
· t(1;19)(q23;p13)
· t(4;11)(q21;q23)
· del (5q)
· monosomy 7
· trisomy 8
· t(8;14)(q24;q32)
· t(8;21)(q22;q22)
· t(8;22)(q24;q11.2)
· t(9;22)(q34;q11.2)
· del (11)(q23)
· t(14;18)(q32;q21)
· t(15;17)(q22;q12)
· inv 16
· t(11;14)(q13;q32)
Time permitting, culture and karyotype peripheral blood cells from him/herself or others to practice the procedures they have learned
Review cases with the directors
Additional procedures from the pathology residents training list if interested (not required)
Attend:
Clinical case conference (see calendar)
Friday noon and other seminars
Literature searches/research project

p:\document\surti\training checklist for hematopath fellow.doc

08/13/2002