Wells' Lab - Publications

Cell Biology - biochemistry and biophysics

P Chen, K Gupta, A Wells (1994). Cell movement elicited by epidermal growth factor receptor requires kinase and autophosphorylation but is separable from mitogenesis. Journal of Cell Biology 124, 547-555.
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P Chen, H Xie, MC Sekar, K Gupta, A Wells (1994). Epidermal growth factor receptor- mediated cell motility: Phospholipase C activity is required, but MAP kinase activity is not sufficient for induced cell motility. Journal of Cell Biology 127, 847-857.
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M Zhang, RK Singh, M-H Wang, A Wells, GP Siegal (1996). Epidermal growth factor modulates cell attachment to hyaluronic acid by the cell surface glycoprotein CD44. Clinical and Experimental Metastasis 14, 268-276. 
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M Zhang, M-H Wang, RK Singh, A Wells, GP Siegal (1997). Epidermal growth factor induces CD44 expression through a novel regulatory element in mouse fibroblasts. Journal of Biological Chemistry 272, 14139-14146. 
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P Chen, J Murphy-Ullrich, A Wells (1996). A role for gelsolin in actuating EGF receptor-mediated cell motility. Journal of Cell Biology 134, 689-698. 
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H Xie, MA Pallero, K Gupta, MF Ware, P Chang, W Witke, DJ Kwiatkowski, DA Lauffenburger, J Murphy-Ullrich, A Wells (1998). EGF receptor regulation of cell- substratum interactions: EGF-induced disassembly of focal adhesions does not require the motility-associated PLCy signaling pathway. Journal of Cell Science 111, 616-625. 
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A Wells, K Gupta, P Chang, S Swindle, A Glading, H Shiraha (1998). Epidermal growth factor receptor-mediated motility in fibroblasts. Microscopy Research and Technique 43, 395-411.
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G Maheshwari, A Wells, LG Griffith, DA Lauffenburger (1999). Crosstalk between EGF and fibronectin receptors affects fibroblast migration and underlying biophysical processes. Biophysical Journal 76, 2814-2823.

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A Glading, P Chang, DA Lauffenburger, A Wells (2000).  EGF receptor activation of calpain is required for fibroblast motility and occurs via and ERK/MAP kinase signaling pathway.  Journal of Biological Chemistry 275, 2390-2398.
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U Marti, A Wells (2000).  The nuclear accumulation of a variant epidermal growth factor receptor (EGFR) lacking the transmembrane domain requires co-expression of a full length EGFR.  Molecular Cell Biology Research Communications 3, 8-14.
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MA Barbieri, R Roberts, A Gumusboga, H Highfield, C Alvarez-Dominguez, A Wells, PD Stahl (2000). Signal transduction and membrane trafficking:  activation of MAP kinase and endocytosis by the EGF receptor requires Rab 5a. Journal of Cell Biology 151, 539-550.
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TB Deb, L Su, L Wong, E Bonvini, A Wells, M David, GR Johnson (2001). EGF receptor kinase-independent signaling by EGF. Journal of Biological Chemistry 276, 1554-15560.
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A Glading, F Uberall, SM Keyse, DA Lauffenburger, A Wells (2001).  Membrane proximal ERK signaling is required for M-calpain activation downstream of EGF receptor signaling. Journal of Biological Chemistry 276, 23341-23348.
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Pathology - tumor progression

BW Hughes, A Wells, Z Bebok, VK Gadi, RI Garver, WB Parker, EJ Sorscher (1995). Sensitization and high level bystander killing of melanoma cells using the human tyrosinase promoter to express the E. coli purine nucleoside phosphorylase gene. Cancer Research 55, 3339-3345.
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H Xie, T Turner, M-H Wang, RK Singh, GP Siegal, A Wells (1995). Prostate carcinoma cell, DU-145, invasiveness in vitro is modulated by EGF receptor signaling. Clinical and Experimental Metastasis 13, 407-419. 
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T Turner, P Chen, LJ Goodly, A Wells (1996). EGF receptor signaling enhances in vivo invasiveness of DU-145 human prostate carcinoma cells. Clinical and Experimental Metastasis 14, 409-418. 
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WB Parker, SA King, PW Allan, LL Bennett, JA Secrist, JA Montgomery, KS Gilbert, WR Waud, A Wells, GY Gillespie, EJ Sorscher (1997). In vivo gene therapy of cancer using E. coli purine nucleoside phosphorylase. Human Gene Therapy, 8 1637-1644. 
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RW Hardy, NSMD Wickramasinghe, A Wells (1997). Fatty acids and breast cancer cell proliferation. Advances in Experimental Medicine and Biology, 422, 57-69. 
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T Turner, M Van Epps-Fung, J Kassis, A Wells (1997). Molecular inhibition of PLCg signaling abrogates DU-145 prostate tumor cell invasion. Clinical Cancer Research 3, 2275-2282. 
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T Turner, W Roberts, G R Pettit, A Wells, A Kraft (1998). Treatment of human prostate cancer with Dolastatin 10, a peptide isolated from a marine shell-less mollusk. Prostate 34, 175-181. 
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H Qin, JD Moellinger, A Wells, LJ Windsor, Y Sun, EN Benveniste (1998). Transcriptional suppression of matrix metalloproteinase-2 gene expression in human astroglioma cells by TNF-a and IFN-g. Journal of Immunology 161, 6664-6673.

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S Khoshyomn, PL Penar, J Rossi, A Bhushan, A Wells, TR Tritton, DL Abamson (1999).  Inhibition of PLC gamma-1 activation blocks glioma cell motility and invasion of fetal rat brain aggregates. Neurosurgery 44, 568-577.

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JA Secrist, WB Parker, PW Allan, LL Bennett, WR Waud, JW Truss, AT Fowler, JA Montgomery, SE Ealick, AH Wells, GY Gillespie, VKGadi, EJ Sorscher (1999).  Gene therapy of cancer: activation of nucleoside prodrugs with E. coli purine nucleoside phosphorylase. Nucleosides and Nucleotides 18, 745-757.

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J Kassis, J Moellinger, H Lo, N Greenberg, H Kim, A Wells (1999).  A role for PLCg-mediated signaling in tumor cell invasion.  Clinical Cancer Research 5, 2251-2260.

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A Wells (2000). Tumor invasion: role of growth factor-induced cell motility Advances in Cancer Research 78, 31-101.
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J Kassis, DA Lauffenburger, T Turner, A Wells (2001).  Tumor invasion as dysregulated cell motility.  Seminars in Cancer Biology 11, 105-119.
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A Wells (2000). The epidermal growth factor receptor ? a new target in cancer therapy. Signal 1, 4-10.

EJ Manos, M Kim, J Kassis, B Chang, A Wells, DA Jones (2001). Dolichol-phosphate-mannose-3 (DPM3)/prostin-1 is a novel phospholipase C-gamma regulated gene negatively associated with prostate tumor invasion. Oncogene 20, 2781-2790.
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Physiology - wound healing and organ morphogenesis

MF Ware, A Wells, DA Lauffenburger (1998). Epidermal growth factor increases fibroblast migration speed but decreases directional persistence in a substratum-dependent manner. Journal of Cell Science 111, 2423-2432.
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H Shiraha, A Glading, K Gupta, A Wells (1999).  Chemokine transmodulation of EGF Receptor signaling:  IP-10 inhibits motility by decreasing EGF-induced calpain activity.  Journal of Cell Biology 146, 243-253.
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H Kim, T Turner, J Kassis, A Wells (1999).  EGF receptor signaling in prostate development.  Histology and Histopathology 14, 1175-1182.
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G Maheshwari, G Brown, DA Lauffenburger, A Wells, LG Griffith (2000).  Cell adhesion and motility depend on nanoscale RGD clustering.  Journal of Cell Science 113, 1677-1686.
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H Shiraha, K Gupta, KA Drabik, A Wells (2000).  Preferential loss of epidermal growth factor receptor results in reduced responsiveness in aging dermal fibroblasts.  Journal of Biological Chemistry 275, 19343-19351.
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A Wells, MF Ware, FD Allen, DA Lauffenburger (1999).  Shapping up for shipping out: PLC signaling of morphology changes in EGF-stimulated fibroblast migration.  Cell Motility and the Cytoskeleton 44, 227-233.
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CS Swindel, KT Tran, TD Johnson, P Banerjee, AM Mayes, LG Griffith, A Wells (2001). Direct activation of the epidermal growth factor receptor by EGF-like repeats of human tenascin-C. Journal of Cell Biology, in press.

DA Lauffenburger, A Wells (2001).  Getting a grip: new insights for cell adhesion and traction. Nature Cell Biology 3, E110-112.
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Signaling and metabolic consequences

DA Lauffenburger, L Chu, A French, F Oehrtman, C Reddy, B Will, A Wells, HS Wiley (1996). Engineering dynamics of growth factors and other therapeutic ligands. Biotechnology and Bioengineering 52, 61-80.
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P Chen, H Xie, A Wells (1996). Mitogenic signaling from the EGF receptor is attenuated by a phospholipase C-g/protein kinase C feedback mechanism. Molecular Biology of the Cell 7, 871-881. 
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M David, L Wong, R Flavell, SA Thompson, A Wells, AC Larner, GR Johnson (1996).
STAT activation by EGF and amphiregulin: requirement for the EGF receptor
kinase, but not for tyrosine phosphorylation sites or JAK1. Journal of Biological
Chemistry 271, 9185-9188.
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C Reddy, A Wells, DA Lauffenburger (1996). Receptor-mediated effects on ligand availability influence relative mitogenic potencies of epidermal growth factor and transforming growth factor a. Journal of Cellular Physiology 166, 512-522. 
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CC Reddy, SK Niyogi, A Wells, HS Wiley, DA Lauffenburger (1996). Re-engineering epidermal growth factor for enhanced potency. Nature Biotechnology 14, 1696-1699. 
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CC Reddy, A Wells, DA Lauffenburger (1994). Proliferative response of fibroblasts expressing internalization-deficient EGF receptors is altered via differential EGF depletion effects. Biotechnology Progress 10, 377-384. 
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R Hardy, KB Gupta, JM. McDonald, J Williford, A Wells (1995) EGF receptor carboxy- terminal domains are required for EGF-induced glucose transport in transgenic 3T3- L1 adipocytes. Endocrinology 136, 431-439. 
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M Van Epps-Fung, K Gupta, J Williford, RW Hardy, A Wells (1996). Epidermal growth factor induces glucose storage in transgenic 3T3-L1 adipocytes overexpressing epidermal growth factor receptors. Diabetes 45, 1619-1625. 
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M Van Epps-Fung, J Williford, A Wells, RW Hardy (1997). Fatty acid-induced insulin resistance in adipocytes. Endocrinology, 138, 4338-4345. 
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M Van Epps-Fung, K Gupta, R Hardy, A Wells (1997). A role for phospholipase C activity in GLUT4-mediated glucose transport. Endocrinology, 138, 5170-5175. 
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CC Reddy, A Wells, DA Lauffenburger (1998). Comparative mitogenic potencies of EGF and TGFa depend on receptor limitation versus ligand-limitation. Medical and Biological Engineering and Computation 36, 499-507. 
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L Wong, TB Debs, A Wells, SA Thompson, GR Johnson (1999).  A differential requirement for the COOH-terminal region of the EGF receptor in Amphiregulin and EGF mitogenic signaling. Journal of Biological Chemistry 274, 8900-8909.
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JM Haugh, A Wells, HS Wiley, DA Lauffenburger (1999).  Effect of epidermal growth factor receptor internalization on regulation of the phospholipase C-g signaling pathway. Journal of Biological Chemistry 274, 8958-8965.
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A Wells (1999).  Molecule in Focus:  EGF receptor.  International Journal of Biochemistry and Cell Biology 31,637-643.  
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JM Haugh, AC Huang, HS Wiley, A Wells, DA Lauffenburger (1999).  Participation of internalized epidermal growth factor receptors in the activation of p21ras.  Journal of Biological Chemistry 274, 34350-34360.
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JM Haugh, A Wells, DA Lauffenburger (2000).  Mathematical modeling of epidermal growth factor receptor signaling through the Phospholipase C pathway:  mechanistics insights and predictions for molecular interventions.  Biotechnology and Bioengineering 70, 225-238.
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RJ Botelho, M Teruel, R Dierckman, R Anderson,  A Wells, JD York, T Meyer, S Grinstein (2000).  Localized changes in phosphatidylinositol-4,5-bisphosphate metabolism during Fcg receptor-mediated phagocytosis. Journal of Cell Biology 151, 1353-1367.
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A Wells (2001).  The EGF receptor signaling system: a model for growth factor receptor signaling.  Hormone Signaling, in press.