Wells' Lab - Angela J. Glading

Scaife 711
Department of Pathology
University of Pittsburgh
Pittsburgh, PA 15261
412-624-0613

aglading+@pitt.edu

Education

Institution Degree Conferred Field of study
Whitworth College, Spokane, WA BS 1997 Biology/Biochemistry
University of Alabama at Birmingham Ph.D Transferred Biology/Biochemistry
University of Pittsburgh  Ph.D anticipated 2001 Biology/Biochemistry

Research Experience

Date Description of research Principal Investigator/Institution
August-December 1997 Research rotation: Determined identity of clones selected from library of insulin responsive rat cDNAs. Joseph Messina Ph.D./UAB
January-March 1998

Research rotation: Studied effect of fatty acid-induced insulin resistance on lipolysis stimulated by beta-adrenergic agonists.

Robert Hardy, Ph.D./UAB
April 1998-present Research rotation/dissertation research: Study of EGF-induced calpain activity in growth factor induced motility. Alan Wells, M.D., D.M.S./UAB & University of Pittsburgh

Publications

1. Wells A., Gupta K., Chang P., Swindle S., Glading A., Shiraha H., Epidermal growth factor receptor-mediated motility in fibroblasts., Microscopy Research and Technique, 43(5):395-411, Dec 1, 1998

2.  Shiraha H., Glading A., Gupta K., Wells A., IP-10 inhibits EGF-induced motility by decreasing EGF receptor-mediated calpain activity., Journal of Cell Biology, 146(1):243-253, July 12, 1999

3.  Glading A., Chang P., Lauffenburger D., Wells A., EGF receptor activation of calpain is required for fibroblast motility and occurs via an ERK/MAP kinase signaling pathway, Journal of Biological Chemistry, 275:2390-2398, Jan 28, 2000

4.  Glading A., Überall F., Keyse S.M., Lauffenburger D.A., Wells A., Membrane-proximal ERK signaling is required for M-calpain activation downstream of EGF receptor signaling., Journal of Biological Chemistry, 276:23341-23348, June 29, 2001

Abstracts

1.   “EGF-Induced Motility Requires Calpain Activation Occurring Downstream of MAP Kinase” Glading A., Chang P., Gupta K., Wells A., 38th ASCB Annual Meeting Abstracts, MCB, 9(suppl.):298a, Nov 1998

2.  “Palmitate Inhibits Insulin Action by Increasing cAMP in Rat Adipocytes” Hardy R., Glading A., Cox E, Diabetes 59th Annual Scientific Sessions, Diabetes 48(suppl.):A251, June 1999

3.   “EGF-Induced Motility Requires Calpain Activation Occurring Downstream of MAP Kinase” Glading A., Gupta K., Chang P., Wells A., FASEB Summer Research Conference: The Calpain System in Health and Disease, June 20-25 1999

4.   “EGF Receptor-Induced M-calpain Activity Downstream of ERK/MAP Kinase is Required for Fibroblast Motility” Glading A., Chang P., Reynolds I.J., Wells A., 39th ASCB Annual Meeting Abstracts (Late Abstracts),  Dec 1999

5.  “The ELR-Negative CXC Chemokines Inhibits EGF-Induced Cell Motility by Limiting Calpain Activation” Shiraha H., Glading A., Wells A., 40th ASCB Annual Meeting Abstracts, MCB , Dec 2000

6.  “EGF Receptor Activates Calpain via ERK Phosphorylation, Not Increased Calcium Flux” Glading A., Reynolds I.J., Wells A., 40th ASCB Annual Meeting Abstracts, MCB, Dec 2000

7.  “Growth factor-induced M-calpain activity is dependent on ERK phosphorylation, but not increased calcium” Glading A., Reynolds I.J., Shiraha H., Wells A., FASEB Summer Research Conference: The Calpain Gene Family in Health and Disease,June 30-July 5 2001