Contributed by Stephen Hastings, MD, Marie DeFrances, MD, PhD, Miroslav Djokic, MD
The patient is a 53 year old male who presented with intermittent fevers, night sweats, and a chronic cough. The patient underwent a PET scan, which showed an enlarged supraclavicular lymph node, a lesion in the left lobe of his liver and bulky retroperitoneal lymphadenopathy. His peripheral blood showed a normocytic normochromic anemia with leukocytosis. The patient underwent excision of the supraclavicular lymph node.
Image 9: CD20; Image 10: CD3; Image 11: CD30; Image 12: CD15; Image 13: MUM-1; Image 14: PAX5; Image 15: OCT2; Image 16: BOB.1; Image 17: EMA; Image 18: EBV ISH (EBER); Image 19: CD2; Image 20: CD4; Image 21: CD5; Image 22: CD8; Image 23: CD20; Image 24: CD3; Image 25: CD10; Image 26: bcl-2
The following immunohistochemical stains were negative in the large cells: LCA, CD45, Alk-1, PD-1, CD7, Beta-F1, TIA1, Granzyme, and AE1/AE3.
Images 27, 28, 29, 30, 31, 32 and 33
Image 34: PCR using BIOMED-2 primers (Framework Region 1)
Image 35: PCR using BIOMED-2 primers (Framework Region 2)
Image 36: PCR using BIOMED-2 primers (Framework Region 3)
Image 37: PCR using BIOMED-2 primers (Kappa)
Image 38: PCR using BIOMED-2 primers (Kappa Deleting Element)