Lane 4 January 1993
Lane 5 May 1993
Lane 6 September 1993
There is a progressive increase in the quantity of monoclonal paraprotein in the gamma region as shown by this high resolution protein electrophoresis.
Immunofixation of the patient's plasma in september 1993 identifies the monoclonal paraprotein as IgM/kappa. The second band migrating toward the anode is fibrinogen.
BONE MARROW BIOPSY
Serum and EDTA plasma were submitted for routine analysis. Protein electorphoresis was performed on an agarose gel and immunofixation was performed by conventional procedures. Quantitative protein measurement was performed by rate nephelometry, an automated laboratory measurement of light scatter in an optical cell following the introduction of an antibody that specifically binds an analyte. The magnitude of the light-scattering signal generated by antigen-antibody complex depends on the wavelength of the incident light, ionic strength of the buffer, antibody affinity to the analyte and concentration solvent with respect to the analyte.