FLOW CYTOMETRY:
INTERPRETATION:
The flow cytometric immunophenotypic studies was not entirely conclusive due to the relatively low viability. The results demonstrate a CD5 negative, CD10 positive monoclonal kappa positive B-cell population. There are also admixed heterogeneous T-cells.
RESULTS:
Cell suspension immunophenotypic studies were performed on liver and two regions were analyzed.
Region 1 represents the small, non-complex cells (16% of the
events).
Region 2 represents the large, non-complex cells (2% of the
events).
VIABILITY: 60%
| B Cells | R1 | R2 | |
|---|---|---|---|
| Antigen | Usual Specificity | % Positive | |
| Kappa+ | B Cell Subset | 14 | 73 |
| Lambda+ | B Cell Subset | 1 | 0 |
| Kappa:Lambda | 14.0 | > 73.0 | |
| CD19+ | B Cell | 19 | 81 |
| CD19+/CD5+ | B Cell Subset | 2 | 3 |
| CD20+ | B Cell | 13 | 68 |
| CD10 (Calla) | B Cell Subset | 15 | 80 |
| T Cells | R1 | R2 | |
|---|---|---|---|
| Antigen | Usual Specificity | % Positive | |
| CD2+ | T Cell | 82 | 17 |
| CD3+ Total | T Cell | 79 | 13 |
| CD5+/CD19- | T Cell | 78 | 12 |
| CD7+ | T Cell | 70 | 15 |
| CD4+/CD8- | T Helper | 49 | 11 |
| CD8+/CD4- | T cytotoxic/suppressor | 26 | 2 |
| CD4+/CD8+ | T Cell Subset | 4 | 2 |
| CD4:CD8 | T Helper:Suppressor ratio | 1.9 | 5.5 |
| CD16, CD56+/CD3+ | T Cell Subset | 3 | 2 |
| CD16, CD56+/CD3- | NK Cell | 2 | 4 |
| Additional Antibodies | R1 | R2 | |
|---|---|---|---|
| Antigen | Usual Specificity | % Positive | |
| CD45+ | Leukocytes | 99 | 96 |
