Flow Cytometry -- Sore Throat and Leukocytosis


FLOW CYTOMETRY:

Results:

Cell suspension immunophenotypic studies were performed on bone marrow aspirate and two regions were analyzed.

Region 1 (R1) represents the small, non-complex cells (7% of the events).

Region 2 (R2) represents the large, non-complex cells (48% of the events).

VIABILITY: 97%

B Cells
Antigen Usual Specificity % Positive (R1) % Positive (R2)
Kappa + B Cell Subset 52 2
Lambda + B Cell Subset 2 2
Kappa:Lambda Ratio 26 1
CD19+ B Cell 76 9
CD19+/CD5+ B Cell Subset 69 3
CD20+ B Cell 45 3
CD10+ (Calla) B Cell Subset 7 6

T Cells
Antigen Usual Specificity % Positive (R1) % Positive (R2)
CD2+ T Cell 18 53
CD3+ Total T Cell 13 2
CD5+/CD19- T Cell 16 11
CD7+ T Cell 16 19
CD4+/CD8- T Helper 6 20
CD8+/CD4- C Cytotoxic/Suppressor 8
CD4+/CD8+ T Cell Subset 1 1
CD4:CD8 Ratio T Helper:Suppressor Ratio 0.75
CD16,CD56+/CD3+ T Cell Subset 1
CD16,CD56+/CD3- NK Cells 3 10

Myeloid
Antigen Usual Specificity % Positive (R1) % Positive (R2)
CD13+/CD33+ Myeloid, monocyte 25 91
CD14+ Myeloid, monocyte 2 18
CD15+ Myeloid 28 43
CD34+ Stem Cell 5 40
CD34+/CD13,33+ Myeloid, monocyte 5 40

Additional Antibodies
Antigen Usual Specificity % Positive (R1) % Positive (R2)
CD45+ Leukocytes 95 98
HLA DR+ B, Myeloid, Activated T 83 95
MPO Myeloid 2 32

Interpretation:

In Region 1, the majority of the cells express CD5 positive/CD19 positive and dim kappa. These findings are consistent with chronic lymphocytic leukemia.

In Region 2, the cells are CD13/CD33 positive, CD2 positive and CD34 positive. These findings are consistent with acute myelogenous leukemia. The expression of CD2 in association with myeloid markers could be used in the follow-up of this patient. The analysis of CD19 combined with CD13 and CD33 indicates that these cells do not express mixed surface markers and two distinct populations are identified by flow cytometric studies.

Final Diagnosis


Case 

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