FLOW CYTOMETRY:
Flow cytometric immunophenotypic studies were performed on bone marrow and demonstrated a population of B-lymphocytes which co-expressed CD19, CD10 and CD34. These cells also were TdT positive and weakly positive for CD22. This phenotype described 25-28% of the total cellularity noted in the marrow specimen and was consistent with acute lymphoblastic leukemia. Cell suspension immunophenotypic studies were performed on bone marrow and one region was analyzed.
| B Cells | R1 | |
|---|---|---|
| Antigen | Usual Specificity | % Positive |
| Kappa+ | B cell subset | 2 |
| Lambda+ | B cell subset | 2 |
| Kappa:Lambda | 1.0 | |
| CD19+ | B cell | 62 |
| CD19+/CD5+ | B cell subset | 1 |
| CD20+ | B cell | 6 |
| CD10+ | B cell subsets | 64 |
| T Cells | R1 | |
|---|---|---|
| Antigen | Usual Specificity | % Positive |
| CD2+ | T cell | 26 |
| CD3+Total | T cell | 22 |
| CD5+/CD19- | T cell | 26 |
| CD7+ | T cell | 19 |
| CD4+/CD8- | T helper | 13 |
| CD8+/CD4- | T cytotoxic/suppressor | 10 |
| CD4+CD8+ | T cell subset | 0 |
| CD4:CD8 | T Helper:suppressor ratio | 1.3 |
| CD16,CD56+/CD3+ | T cell subset | 0 |
| CD16,CD56+/CD3- | NK cell | 4 |
| Myeloid | R1 | |
|---|---|---|
| Antigen | Usual Specificity | % Positive |
| CD13,CD33+ | Myeloid, monocyte | 6 |
| CD14+ | Myeloid, monocyte | 1 |
| CD15+ | Myeloid | 5 |
| CD34+ | Stem cell | 69 |
| CD34+/CD13,33+ | Immature myeloid, monocyte | 3 |
| Additional Antibodies | R1 | |
|---|---|---|
| Antigen | Usual Specificity | % Positive |
| CD45+ | Leukocytes | 30 |
| HLA DR+ | B, Myeloid, Activated T | 78 |
| CD22+ | B cells | 30 |
| TdT+ | Lymphoblasts(myeloblasts) | 77 |
| Tdt+/Cyto CD3+ | T Lymphoblasts | 0 |
| Myeloperoxidase+ | Myeloid | 1 |
| CD19+/CD13+,33+ | 2 | |
| CD7+/CD13+33+ | 1 | |
| CD19+/CD34+ | Lymphoid blasts | 68 |
MOLECULAR CYTOGENETIC ANALYSIS
