MOLECULAR GENETICS STUDIES:
Molecular studies were performed to assess immunoglobulin heavy chain gene rearrangement (Southern blot and PCR) and T cell receptor beta chain gene rearrangement (Southern blot). Germline alleles only were demonstrated using both IgH and TCR-beta chain gene probes. PCR analysis using V region framework 3 and consensus J region primers disclosed a polyclonal amplification pattern without a clonal band. Immunoglobulin heavy chain and T-cell receptor beta chain gene rearrangement were not demonstrated.
