Jean J. Latimer, Assistant Professor
PhD, Roswell Park Cancer Institute
State University of NY
Email: latimerj@pitt.edu, latimerjj@upmc.edu
HomePage: http://www.upci.upmc.edu/labs/latimer/index.html
Research Interests:
Dr. Jean Latimer's laboratory is engaged in human breast tissue engineering and breast cancer research. Her laboratory has developed a novel method of establishing long lived primary cultures from non-diseased breast tissue as well as breast tumors (stages 0-IV). From these robust primary cultures her laboratory has created over 100 cell lines without the use of exogenously transforming agents.
Dr. Latimer's laboratory is using this unique tissue engineering methodology to investigate the role of Nucleotide Excision Repair in Breast Cancer etiology. In addition her most recent funding is to determine how stem cells in the breast may contribute to racially disparate types of breast cancer.
Grants:
"Biomarkers of invasiveness in racially disparate populations using an isogenic and progressive model of human DCIS"
Principal Investigator: Latimer, J.J.
Agency: Komen for the Cure
Period: May 1, 2007-April 31, 2009
The major aim of this grant is to use 3 unique DCIS (and matching contralateral breast) cell lines to establish biomarkers of pre-invasive breast cancer aggressivenss using expression microarray technology and xenograft mice.
"Quantitative proteomics of nuclear matrix proteins in novel human ductal carcinoma in situ model systems"
Principal Investigators: (Day,B. and Latimer, J.J.)
Agency: Department of Defense (synergy award) ($700,000)
Period: Dec. 1, 2008-Nov. 31, 2010
The major aim of this grant is to test the hypothesis that detectable differences exist between invasive and noninvasive DCIS at the protein level, particularly in the nucleus, and these differences are distinct from those demonstrated at the nucleic acid level.
Recent Publications
Grant, S.G., Das, R., Cerceo, C.M., Rubinstein, W.A., Latimer, J.J. (2007) Elevated levels of somatic mutation in a manifesting BRCA1 mutation carrier. Pathology Oncology Research, in press.
Donovan, M., Miles, T.D., Latimer, J.J., Grant, S.G. Talbott, E., Sasco, A.J., and Davis, D.L. (2006) Association between biomarkers of environmental exposure and increased risk of breast cancer. Nature Rev. Cancer 6: c1
Rubinstein, W.S., Latimer, J.J., Sumkin, J., Huerbin, M.B.. Grant, S.G. and, Vogel, V. G. (2006) Prospective screening study of 0.5 Tesla dedicated magnetic resonance imaging for the detection of breast cancer in young high-risk women, BMC WomenĄŻs Health 6(10) online.
Donovan, M., Miles, T.D., Latimer, J.J., Grant, S.G. Talbott, E., Sasco, A.J., and Davis, D.L. (2006) Association between biomarkers of environmental exposure and increased risk of breast cancer. Nature Rev. Cancer 6: c1.
Kelly, C.M. and Latimer, J.J. (2005) Unscheduled DNA synthesis: a functional assay for global genomic nucleotide excision repair. Methods in Molecular Biology 291: 303-320.
Johnson, J.M., and Latimer, J.J. (2005) Analysis of DNA repair using transfection-based host cell reactivation. Methods in Molecular Biology 291: 321-335.
Latimer, J.J., Rubinstein, W.S., Johnson, J.M., Kanbour-Shakir, A., Vogel, V.G., and Grant, S.G. (2005) Haploinsufficiency for BRCA1 is associated with normal levels of DNA nucleotide excision repair in breast tissue and blood lymphocytes. BMC Medical Genetics 6: 26.
Latimer, J.J., Nazir, T., Flowers, L.C., Forlenza, M.J., Beaudry-Rodgers, K., Kelly, C.M., Conte, J.A., Shestak, K., Kanbour-Shakir, A., Grant, S.G. (2003) Unique tissue-specific level of DNA nucleotide excision repair in primary human mammary epithelial cultures. Experimental Cell Research 291:111-121.
Draper, D., Kush, M.J., Donohoe, W., Janosky, J., Latimer, J.J., Heine R.P. (2001) Preterm premature rupture of membranes (pPROM) without labor is not associated with increased levels of matrix metalloproteinase-9 (MMP-9) protein. Prenatal and Neonatal Medicine 6:1-8.
Grant, S.G., Wenger, S.L., Latimer, J.J., Thull D, Burke, L.W. (2000) Analysis of genomic instability using multiple assays in a patient with Rothmund-Thomson syndrome. Clinical Genetics 58:209-215.
Forlenza, M., Latimer, J.J., Baum, A. (2000) The effects of stress on DNA repair capacity. Psychology & Health 15:881-891
